Reporter

Part:BBa_K612003:Design

Designed by: Michael Simpson   Group: iGEM11_Alberta   (2011-09-28)


Hygromycin B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 17
    Illegal EcoRI site found at 659
    Illegal XbaI site found at 2169
    Illegal PstI site found at 11
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 17
    Illegal EcoRI site found at 659
    Illegal PstI site found at 11
    Illegal PstI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 17
    Illegal EcoRI site found at 659
    Illegal BamHI site found at 1457
    Illegal BamHI site found at 2175
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 17
    Illegal EcoRI site found at 659
    Illegal XbaI site found at 2169
    Illegal PstI site found at 11
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 17
    Illegal EcoRI site found at 659
    Illegal XbaI site found at 2169
    Illegal PstI site found at 11
    Illegal PstI site found at 749
    Illegal NgoMIV site found at 2086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1806


Design Notes

N/A


Source

This part was obtained from the Fungal Genetic Stock Center and Subsequently altered to remove a internal BsaI cut site.

References